Journal: Journal of Cell Communication and Signaling

Article Title: METTL13 facilitates cell growth and metastasis in gastric cancer via an eEF1A/HN1L positive feedback circuit

doi: 10.1007/s12079-022-00687-x

Figure Lengend Snippet: METTL13 modulated HN1L expression in GC. a Genes that showed positively association with METTL13 were surveyed by COEXPEDIA database ( http://www.coexpedia.org/ ). qRT-PCR was performed to test the expression of METTL13 and HN1L in METTL13 knockdown cells ( b ) and METTL13 overexpression cells ( c ). d HN1L or METTL13 mRNA expression was upregulated in GC tissues than that in the normal tissues based on GEPIA online analysis for TCGA database. e Positive correlation between METTL13 and HN1L expression at mRNA levels were analyzed using GEPIA online tools. f Western blotting was applied to test HN1L protein level upon METTL13 knockdown or overexpression in GC cells. g CCK-8 assays showed that cell viability caused by overexpression of METTL13 was partially attenuated by introducing siRNA targeting HN1L into SCG7901 cells. Data are given as mean ± SD. * P < 0.05; ** P < 0.01

Article Snippet: The primary antibodies used in this study are as follows: METTL13 (#ab186002, Abcam), HN1L (#ab200571, Abcam), eEF1A (#2551, Cell Signaling Technology), β-actin (#81178, Santa Cruz Biotechnology).

Techniques: Expressing, Quantitative RT-PCR, Knockdown, Over Expression, Western Blot, CCK-8 Assay

Journal: Journal of Cell Communication and Signaling

Article Title: METTL13 facilitates cell growth and metastasis in gastric cancer via an eEF1A/HN1L positive feedback circuit

doi: 10.1007/s12079-022-00687-x

Figure Lengend Snippet: eEF1A was involved in the regulation of HN1L by METTL13. a Knockdown efficacy for eEF1A1 or eEF1A2 was verified by qRT-PCR in AGS and MGC803 cells. b HN1L expression was analyzed by qRT-PCR in eEF1A1 or eEF1A2 knockdown cells. c Mutation site of eEF1A1/eEF1A2. d mRNA level of eEF1A1 or eEF1A2 was tested by qRT-PCR in SGC7901 cells transfected with pENTER-eEF1A1 or pENTER-eEF1A2 plasmid. And overexpression of eEF1A1 or eEF1A2 led to expression upregulation of HN1L by qRT-PCR analysis. No obvious change of HN1L expression was observed between wild type eEF1A and eEF1A K55A mutant cells. e Western blotting indicated that HN1L was decreased when eEF1A1 or eEF1A2 was silenced. f Western blot results were shown, indicating both wild-type and K55A mutant eEF1A upregulated HN1L expression. g Knockdown of eEF1A could partially offset the upregulation of HN1L in METTL13-overexpressed cells. Data are given as mean ± SD. * P < 0.05; ** P < 0.01

Article Snippet: The primary antibodies used in this study are as follows: METTL13 (#ab186002, Abcam), HN1L (#ab200571, Abcam), eEF1A (#2551, Cell Signaling Technology), β-actin (#81178, Santa Cruz Biotechnology).

Techniques: Knockdown, Quantitative RT-PCR, Expressing, Mutagenesis, Transfection, Plasmid Preparation, Over Expression, Western Blot

Journal: medRxiv

Article Title: SARS-CoV-2 seroprevalence in Kaduna State, Nigeria during October/November 2021, following three waves of infection and immediately prior to detection of the Omicron variant

doi: 10.1101/2021.12.21.21268166

Figure Lengend Snippet: Daily new confirmed COVID-19 cases per million people in Nigeria, 7-day rolling average, 4 March 2020 to 30 November 2021. (Source: Our World In Data .)

Article Snippet: The CE-marked Biopanda COVID-19 IgM/IgG Rapid Test Kit was used to screen serum for antibodies, following the manufacturers’ instructions.

Techniques:

Journal: PLoS ONE

Article Title: The Accuracy of Diagnostic Tests for Lyme Disease in Humans, A Systematic Review and Meta-Analysis of North American Research

doi: 10.1371/journal.pone.0168613

Figure Lengend Snippet: Thirteen studies (78 lines of data) evaluating a two-tier serological test protocol summarized by the stage of Lyme disease tested using a hierarchical logistic regression model in Stata 13 or the range of sensitivity and specificity data presented in the paper when less than four lines of data were available for meta-analysis.

Article Snippet: , MarDx Lyme disease (IgG) Marblot strip test system FDA/HC , 1 (2) , 45.2–88.9 P , 100.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: The Accuracy of Diagnostic Tests for Lyme Disease in Humans, A Systematic Review and Meta-Analysis of North American Research

doi: 10.1371/journal.pone.0168613

Figure Lengend Snippet: Eight studies (33 lines of data) evaluating different immunoblots (2nd tier tests) at all stages of Lyme disease using hierarchical logistic regression models or the sensitivity and specificity data when less than four lines of data were available for meta-analysis.

Article Snippet: , MarDx Lyme disease (IgG) Marblot strip test system FDA/HC , 1 (2) , 45.2–88.9 P , 100.

Techniques: Western Blot, Dot Blot, Stripping Membranes

Journal: PLoS ONE

Article Title: The Accuracy of Diagnostic Tests for Lyme Disease in Humans, A Systematic Review and Meta-Analysis of North American Research

doi: 10.1371/journal.pone.0168613

Figure Lengend Snippet: Summary of agreement between tests reported in eight studies (10 lines of data) that examined two-tier serology testing compared to a different test or two-tier protocol.

Article Snippet: , MarDx Lyme disease (IgG) Marblot strip test system FDA/HC , 1 (2) , 45.2–88.9 P , 100.

Techniques: Stripping Membranes, Enzyme-linked Immunosorbent Assay, Amplification

Journal: PLoS ONE

Article Title: The Accuracy of Diagnostic Tests for Lyme Disease in Humans, A Systematic Review and Meta-Analysis of North American Research

doi: 10.1371/journal.pone.0168613

Figure Lengend Snippet: Summary or range of agreement reported in 14 studies (51 lines of data) evaluating different tests paired against each other and tested on samples meeting the clinical definition of Lyme disease or a test panel.

Article Snippet: , MarDx Lyme disease (IgG) Marblot strip test system FDA/HC , 1 (2) , 45.2–88.9 P , 100.

Techniques: Enzyme-linked Immunosorbent Assay, Stripping Membranes, Dot Blot, Western Blot, Peptide ELISA

Journal: PLoS ONE

Article Title: The Accuracy of Diagnostic Tests for Lyme Disease in Humans, A Systematic Review and Meta-Analysis of North American Research

doi: 10.1371/journal.pone.0168613

Figure Lengend Snippet: Summary of the sensitivity and specificity of different testing options for early Lyme disease (stage 1) patients.

Article Snippet: , MarDx Lyme disease (IgG) Marblot strip test system FDA/HC , 1 (2) , 45.2–88.9 P , 100.

Techniques: Enzyme-linked Immunosorbent Assay, Peptide ELISA, Western Blot

Journal: Viruses

Article Title: Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2

doi: 10.3390/v12060582

Figure Lengend Snippet: Specifications of the commercially available rapid serological tests for laboratory diagnosis of COVID-19.

Article Snippet: Phamatech laboratories and diagnostics , COVID-19 “Coronavirus” IgG/IgM Rapid Test Kit , 2278 , Detection of IgG and IgM antibodies , Utilizes nucleocapsid protein (N-protein) as the binding antigen , IgG: Sensitivity: 100% Specificity: 98.0% Accuracy: 98.6% IgM: Sensitivity: 85.0% Specificity: 96.0% Accuracy: 92.9% , Registered with the FDA.

Techniques: Biomarker Discovery, Infection, Recombinant, Labeling, Immunochromatographic Assay, Virus, Diagnostic Assay, Binding Assay, Antigen Assay